Vitamin D2, also known as ergocalciferol, is a form of Vitamin D available in concentrations of 100,000 IU/g or 500,000 IU/g as Cold Water Soluble (CWS) formulations. It is synthesized from ergosterol through ultraviolet irradiation and serves as an essential nutrient for human health. Vitamin D2 plays a crucial role in regulating calcium and phosphate metabolism, thereby supporting bone health and mineralization. Beyond its skeletal benefits, Vitamin D2 is involved in modulating immune function and reducing inflammation. Its Cold Water Soluble form enhances its bioavailability and suitability for incorporation into various food products and dietary supplements. Vitamin D2 is particularly important for individuals at risk of deficiency due to limited sunlight exposure or dietary intake, highlighting its significance in maintaining overall health and mitigating the risk of musculoskeletal disorders.